eldorado.tu-dortmund.de/server/api/core/bitstreams/b10ede4e-b815-4ced-922e-f9c3aa0cbfbd/content
Microsoft Word - Vazquez-Moreno21-06proof1.doc
with five bed volumes of 0.5 M Na2SO4, 10 mM MOPS, pH 7.6 (Buffer A) and samples of 5 mL of porcine serum (adjusted to 0.5 M Na2SO4, 10 mM MOPS, pH 7.6) loaded onto column. The gel was washed with Buffer [...] were eluted with 10 mM MOPS Buffer, pH 7.6 (Buffer B). Following elution, HA- Sepharose was cleaned with two bed volumes of 4 M guanidine hydrochloride (guanidine- HCl), pH 7.6; washed with five bed volumes [...] Denmark). Gels were packed in columns (4 x 1 cm), equilibrated with PBS, pH 7.2 and loaded with 10 mg of isolated Igs fraction (in PBS, pH 7.2) from hydrophobic interaction chromatography. Unbound proteins …
